Path: utzoo!mnetor!uunet!munnari!murdu!sam From: sam@murdu.OZ (Sam Ganesan) Newsgroups: sci.bio Subject: Re: Antisense-RNA-derived mutants Message-ID: <1366@murdu.OZ> Date: 8 May 88 08:23:56 GMT References: <1784@aecom.YU.EDU> Reply-To: sam@murdu.UUCP (Sam Ganesan) Organization: Microbiology Dept, Melbourne Uni, Australia Lines: 32 In article <1784@aecom.YU.EDU> diaz@aecom.YU.EDU (Dizzy Dan) writes: >I am interested in generating mutant strains of E. coli in two genes >I've been working with. I don't want to mutagenize and hunt for mutants >(yuck, what a mess!). Hey Dan, Mutagenising and screening for mutants is not that much of a mess if you know what kind of a mutant you are looking for. Which are genes you are working with. I have been generating spontaneous and oligonucleotide directed mutants in the E. coli gene tyrR and it has not been easy I must admit but has not been that bad either. > The method I use should be simple, effective, and >specific to the genes in question. What if I put an antisense code >for the gene(s) on a plasmid and transform. The antisense message would >then prevent translation of the sense message, right? I'd appreciate some >advice, although, as always, Dan Davison may not respond. I realise antisense RNA was touted as the means of regulation a few years ago but it has quite a few problems. If you really need some references send me a note and I probably can dig up some both for and against. Just out of curiosity, which genes are you working with and what kind of mutants do you want? Sam Ganesan ******************************************************************************* E - mail : ACSnet: sam@murdu.mu.oz JANET: sam%murdu.mu.oz@uk.ac.ukc ARPA : sam%murdu.mu.oz.au@uunet.uu.net sam%murdu.mu.oz@uk.ac.ean-relay UUCP : {uunet,pyramid}!munnari!murdu.mu.oz.au!sam Snail : Sam Ganesan, Microbiology Dept,Melbourne University, Parkville, Victoria 3052, Australia. ******************************************************************************