Relay-Version: version B 2.10 5/3/83; site utzoo.UUCP Path: utzoo!utgpu!water!watmath!uunet!ig!daemon From: daemon@ig.UUCP Newsgroups: bionet.molbio.news Subject: CSLG: COMMENTARY: From Ellis Golub (3) Message-ID: <4261@ig.ig.com> Date: Tue, 1-Dec-87 14:41:32 EST Article-I.D.: ig.4261 Posted: Tue Dec 1 14:41:32 1987 Date-Received: Sat, 5-Dec-87 13:17:45 EST Sender: daemon@presto.ig.com Lines: 21 From: Sunil MaulikComputer Applications in the Sequencing of Large Genomes What will we be searching for in the new, large databases? One use for total genomic DNA will be to locate genomic sequences for isolated cDNA. Thus, after sequencing the cDNA for a message of interest, one would usually like to obtain the genomic sequence, including the structure of the gene and the upstream and downstream regulatory elements. Rather than having to fish a genomic clone out of a library, as is the current practice, it will be possible to find the gene, along with possible pseudogenes, in the database. Indeed, from what is now known, one might only need a small amount of cDNA sequence to deduce the entire message sequence and genomic structure from the database. In addition, the chromosomal location of the gene will also be known, and the possible relationship of the gene to genetically mapped heritable diseases will also be evident. The rapid availability of such information could well justify the difficulty and expense of building the database. -------