Path: utzoo!utgpu!watmath!iuvax!bionet!agate!codon3.berkeley.edu!ladasky From: ladasky@codon3.berkeley.edu (John Ladasky;1021 Solano No. 2;528-8666) Newsgroups: bionet.general Subject: HELP! 3H-tagged SV40? Message-ID: <1989Oct2.221028.17141@agate.berkeley.edu> Date: 2 Oct 89 22:10:28 GMT Reply-To: ladasky@codon3.berkeley.edu.UUCP (John Ladasky) Organization: University of California, Berkeley Lines: 26 Hi, remember me? I'm the "lowly undergraduate" from last year's debate regarding publication of scientific results, peer review, etc. I have run into a bit of a problem which may be familiar to some of you from your grad-school experiences... a combination of time pressure and resource limi- tations. You see, I'm planning experiments which require the preparation of tritiated thymidine-labeled SV40. I spent last semester doing cell culture, and I intended to do experiments this semester. Only - oops - it seems that something (like an enzyme) slipped past my phenol extraction, and now all of my DNA is nicked. And hey, wouldn't you know it, I'm graduating in December. I suppose that I could rewind the DNA with a topoisomerase, and ligate it back together... but it's important that I have DNA that has a linking number in the physiological range. Even if this were feasible, I imagine that learning about this and experimenting with it would take several weeks. So, here's my idea; my grad-student supervisor says that she has not seen any commercial source of tritiated thymidine-labeled SV40 - but does any one else know otherwise? I would be most grateful for this information. Please email to: ladasky@enzyme.berkeley.edu (internet). T CROSS POLICE LINE DO NOT CROSS POLICE LINE DO NOT CROSS POLICE LINE DO NOT CR _______________________________________________________________________________ "Do unto others as you would like - John J. Ladasky ("ii") to do unto them. " Richard Bach (ladasky@enzyme.berkeley.edu)