Path: utzoo!utgpu!watmath!iuvax!bionet!agate!codon3.berkeley.edu!ladasky
From: ladasky@codon3.berkeley.edu (John Ladasky;1021 Solano No. 2;528-8666)
Newsgroups: bionet.general
Subject: HELP!  3H-tagged SV40?
Message-ID: <1989Oct2.221028.17141@agate.berkeley.edu>
Date: 2 Oct 89 22:10:28 GMT
Reply-To: ladasky@codon3.berkeley.edu.UUCP (John Ladasky)
Organization: University of California, Berkeley
Lines: 26


	Hi, remember me?  I'm the "lowly undergraduate" from last year's
debate regarding publication of scientific results, peer review, etc.  I have
run into a bit of a problem which may be familiar to some of you from your
grad-school experiences... a combination of time pressure and resource limi-
tations.

	You see, I'm planning experiments which require the preparation of
tritiated thymidine-labeled SV40.  I spent last semester doing cell culture,
and I intended to do experiments this semester.  Only - oops - it seems that
something (like an enzyme) slipped past my phenol extraction, and now all of
my DNA is nicked.  And hey, wouldn't you know it, I'm graduating in December.

	I suppose that I could rewind the DNA with a topoisomerase, and 
ligate it back together... but it's important that I have DNA that has a
linking number in the physiological range.  Even if this were feasible, I
imagine that learning about this and experimenting with it would take several
weeks.  So, here's my idea; my grad-student supervisor says that she has not
seen any commercial source of tritiated thymidine-labeled SV40 - but does any
one else know otherwise?  I would be most grateful for this information.
Please email to: ladasky@enzyme.berkeley.edu (internet).

T CROSS POLICE LINE DO NOT CROSS POLICE LINE DO NOT CROSS POLICE LINE DO NOT CR
_______________________________________________________________________________
"Do unto others as you would like		       - John J. Ladasky ("ii")
 to do unto them. " Richard Bach		  (ladasky@enzyme.berkeley.edu)