Path: utzoo!utgpu!water!watmath!uunet!ig!bionet-20.bio.net!ABELSON.WESTAWAY
From: ABELSON.WESTAWAY@BIONET-20.BIO.NET (SHAWN WESTAWAY)
Newsgroups: bionet.molbio.proteins
Subject: Determination of Protein pI?
Message-ID: <12411967156.18.ABELSON.WESTAWAY@BIONET-20.ARPA>
Date: 5 Jul 88 21:31:56 GMT
Sender: daemon@presto.ig.com
Lines: 13


Does anyone out there know of a simple and fast method for the
experimental determination of an already purified protein's
isoelectric point, without buying or constructing unusual PAGE
apparatus?  Most of the recent methods I have seen apply to
separating similar proteins away from each other, and involve
construction of different apparatuses, or purchase of buffer
gradient chemicals.  Also, can the pI of a protein be determined
under native conditions, or must it be denatured during the process?

Thanks, reply to ABELSON.WESTAWAY, c/o BIONET-20.ARPA
Shawn Westaway
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