Path: utzoo!utgpu!water!watmath!uunet!ig!daemon From: WOOLFORD.THOMPSON@BIONET-20.ARPA Newsgroups: bionet.molbio.methds-reagnts Subject: Oligo Hyb Conditions Message-ID: <6538@ig.ig.com> Date: 2 Jun 88 00:43:12 GMT Sender: daemon@presto.ig.com Lines: 11 From: JOHN R. THOMPSONI recall that a particular salt (the name of which escapes me) is advantageous to add to hybridizations involving oligomers. It seems this salt minimizes GC bias and makes the Tm strictly dependent on length thereby decreasing false signals from sequences with one or two mismatches. Can anyone give me a pointer to this? Thanks, JRT -------