Path: utzoo!utgpu!water!watmath!uunet!ig!daemon
From: WOOLFORD.THOMPSON@BIONET-20.ARPA
Newsgroups: bionet.molbio.methds-reagnts
Subject: Oligo Hyb Conditions
Message-ID: <6538@ig.ig.com>
Date: 2 Jun 88 00:43:12 GMT
Sender: daemon@presto.ig.com
Lines: 11

From: JOHN R. THOMPSON 

I recall that a particular salt (the name of which escapes me) is 
advantageous to add to hybridizations involving oligomers.  It seems this 
salt minimizes GC bias and makes the Tm strictly dependent on length thereby 
decreasing false signals from sequences with one or two mismatches.  Can 
anyone give me a pointer to this?

Thanks, JRT

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